![Figure 2. Loss of p57 results in a proliferative defect, induction of apoptosis, and excessive phosphorylation of Rb in thymocytes. (A) Representative flow cytometric histograms for BrdU staining in DN3 and DN4 thymocytes from Lck-Cre/p57[+]/+ and Lck-Cre/p57[+]/F mice injected with BrdU at 8 weeks of age. (B) Determination of the proportion of cells positive for BrdU as in panel A. Data are means ± SD for 4 mice. *P < .05, ***P < .005. (C) Representative histograms for annexin V staining in DN3 and DN4 thymocytes from Lck-Cre/p57[+]/+ and Lck-Cre/p57[+]/F mice at 8 weeks of age. (D) Determination of the proportion of cells positive for annexin V staining as in panel C. Data are means ± SD for 4 mice. ***P < .005. (E) IB analysis of phosphorylated Rb in DN3 thymocytes from Lck-Cre/p57[+]/+ and Lck-Cre/p57[+]/F mice at 8 weeks of age. HSP70 was examined as a loading control. (F-G) RT and real-time PCR analysis of Mcm3, Cdc6, and Cdt1 mRNAs (F) as well as of ATM and ASPP1 mRNAs (G) in DN3 thymocytes from Lck-Cre/p57[+]/+ and Lck-Cre/p57[+]/F mice at 8 weeks of age. Normalized data are expressed relative to the corresponding value for control mice and are means ± SD for 3 mice. ***P < .005.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/123/22/10.1182_blood-2013-10-532390/4/3429f2.jpeg?Expires=1722445963&Signature=m~YapcqCALHYgCg5rnE8clEE-RwHOyFNaHki-iMvUtjSdadZPMNs2us76h3Tc44PDsQv8UjBlc4Wwr6z38AX2REqIW0iVAcIQlMVt0YvgnhiSczOgKaIJ2SMKoz~eLzqz40EipbSAKzLDp-wP8mv7XqitJEn7mY3Wi1dUPj6lpQ06TWWtfAnoQ7R9JmBA6dGp4PbhfZOI586Rs5lxfa-lTnxVul3a~2mTn~pgqr8~4RK2FDTOpgpUA-89Jwq-jSdKSowHeQ5VT07E0UC6y287-cStpVvZaFxUgArwFRyo6MKoJm1wrzUxd2wDAbH92BIhho~dphxGAa0X4UqcsRIIQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Loss of p57 results in a proliferative defect, induction of apoptosis, and excessive phosphorylation of Rb in thymocytes. (A) Representative flow cytometric histograms for BrdU staining in DN3 and DN4 thymocytes from Lck-Cre/p57[+]/+ and Lck-Cre/p57[+]/F mice injected with BrdU at 8 weeks of age. (B) Determination of the proportion of cells positive for BrdU as in panel A. Data are means ± SD for 4 mice. *P < .05, ***P < .005. (C) Representative histograms for annexin V staining in DN3 and DN4 thymocytes from Lck-Cre/p57[+]/+ and Lck-Cre/p57[+]/F mice at 8 weeks of age. (D) Determination of the proportion of cells positive for annexin V staining as in panel C. Data are means ± SD for 4 mice. ***P < .005. (E) IB analysis of phosphorylated Rb in DN3 thymocytes from Lck-Cre/p57[+]/+ and Lck-Cre/p57[+]/F mice at 8 weeks of age. HSP70 was examined as a loading control. (F-G) RT and real-time PCR analysis of Mcm3, Cdc6, and Cdt1 mRNAs (F) as well as of ATM and ASPP1 mRNAs (G) in DN3 thymocytes from Lck-Cre/p57[+]/+ and Lck-Cre/p57[+]/F mice at 8 weeks of age. Normalized data are expressed relative to the corresponding value for control mice and are means ± SD for 3 mice. ***P < .005.
