Endothelial BAMBI is responsible for the defect in thrombus stability in vivo. (A) Representative composite fluorescence and brightfield images of laser-induced thrombus formation in arterioles of Bambi^+/+ and Bambi^−/− mice receiving BMCs from Bambi^−/− and Bambi^+/+ mice, respectively. Scale bar represents 10 µm. (B) IFI is depicted (AU) as a function of time after the injury. Distribution of the time to maximal thrombus size (C) and maximal thrombus size expressed in IFI AU (D) in Bambi^+/+/Bambi^−/− and Bambi^−/−/Bambi^+/+ mice. (E) Thrombus stability was assessed by determining the time for each thrombus after reaching maximal size to reduce by 50% of maximal IFI. See supplemental Videos 7-8 for better visualization of the differences in thrombus stability between the different animal groups. Each symbol represents 1 thrombus: Bambi^+/+/Bambi^−/− (n = 19) and Bambi^−/−/Bambi^+/+ mice (n = 16). Horizontal lines intersecting data sets represent the median. Statistical analysis was performed using the unpaired Mann-Whitney test. *P < .05.