Bambi^−/− mice display normal platelet function. (A-D) Representative aggregation traces of washed platelets isolated from Bambi^+/+ and Bambi^−/− mice and stimulated with the indicated concentrations of agonists. Light transmission was recorded on a Chrono-log 2 channel aggregometer over 5 minutes. (E) Bar graphs of results obtained by aggregometry. Results are given as the mean percentage of aggregation ± SEM (n ≥ 3 mice for each condition). Statistical analysis was performed using the unpaired Student t test (P > .05). (F-G) Flow cytometry analysis of α_IIbβ₃ integrin activation (F) and P-selectin expression (G) in response to the indicated concentrations of agonists in platelets from Bambi^+/+ (n = 8) and Bambi^−/− mice (n = 11). Data represent mean fluorescence intensities ± SEM. Statistical analysis was performed using the unpaired Student t test (P > .05). (H) Whole blood from Bambi^+/+ and Bambi^−/− mice was perfused over a collagen-coated surface (0.1 mg/mL) at a shear rate of 1500 s⁻¹. (Left) Representative fluorescence images of aggregate formation on collagen after 3 minutes of perfusion time. Scale bar equals 60 µm. (Right) Surface covered by fluorescently labeled platelets expressed as the percentage of the total surface observed was calculated at the indicated perfusion times. Data represent mean ± SEM (n > 6 for each group). Statistical analysis was performed using the unpaired Student t test (P > .05).