Genetic ablation of Bambi leads to increased tail-bleeding time. (A) PCR was performed on DNA isolated from ear biopsies using previously described primers³⁵  leading to the amplification of the wild-type allele (top; 225bp), and the Bambi-deleted allele (bottom; 316bp). (B) RT-PCR analysis of BMCs isolated from wild-type and Bambi^−/− mice using primers specific to Bambi transcript (1:587 bp; 2:329 bp; 3:812 bp) or Gapdh (Ctrl; 452bp). (C-D) Analysis of tail-bleeding experiments on Bambi^+/+Bambi^+/− and Bambi^−/− mice. (C) Graph showing the time to cessation in bleeding for all animals according to genotype. The percentage of mice that were still bleeding after 12 minutes (dotted line) was significantly higher in Bambi-deficient mice compared with wild-type littermates. Statistical analysis was performed using the χ² test for linear trend (P < .02). Each symbol represents 1 animal. (D) Bambi-deficient mice exhibited a significant increase in blood loss (determined after 10 minutes). Each symbol represents 1 animal. Horizontal lines intersecting data sets represent the median. Statistical analysis was performed using ANOVA (Kruskal-Wallis). *P < .05.