Genetic analysis of 2bF8LV-transduced hCB transplantation recipients. (A) PCR analysis of the 2bF8 transgene. Genomic DNA was purified from peripheral leukocytes. Leukocytes from NSG mice and a normal human individual were used as controls. 2bF8 transgenic mouse DNA was used as a positive control for the 2bF8 transgene. Shown is one representative experiment that was performed 3 times. Wild-type (WT) mouse FVIII and human glyceraldehyde 3-phosphate dehydrogenase (hGAPDH) were used as internal controls. (B) qPCR quantified the average copy number of the 2bF8 transgene per cell in 2bF8LV-transduced hCB transplantation recipients. Peripheral leukocyte–derived genomic DNA was used to analyze 2bF8 proviral DNA with normalization to hGAPDH. Bars represent mean ± SD. For individual mice analyzed more than once over the course of study, the average copy number was calculated. Genomic DNA from NSG mice was used as a control. Data are summarized from 2 trials of xenotransplants.