Human cell chimerism in NSG and NSGF8KO mice that received 2bF8LV-transduced hCB CD34⁺cells. Blood samples were collected from tail bleeds. Human platelets were identified by a mouse anti-human glycoprotein Ib alpha chain (GPIbα) antibody AP1. Mouse platelets were stained with a rat anti-mouse CD41 (GPIIb) antibody. Human leukocytes were identified by mouse anti-human CD45 antibody, and mouse leukocytes were identified by rat anti-mouse CD45 antibody. (A) Representative flow cytometric analysis of platelets in peripheral blood of NSG and NSGF8KO recipients at 3 weeks after transplantation. (B) Representative flow cytometric analysis of leukocytes in peripheral blood of NSG and NSGF8KO recipients between 5 and 7 weeks after transplantation. (C) Human platelet chimerism in NSG and NSGF8KO recipients. Samples from NSG mice and normal human individuals were used as controls. Data are summarized from 10 trials of xenotransplants. (D) Human leukocyte chimerism in NSG and NSGF8KO recipients. Samples from NSG mice and normal human individuals were used as controls. Data are summarized from 10 trials of xenotransplants. (E) Time course of human platelet production in NSG recipients. Data are summarized from 2 trials of xenotransplants (n = 4 to 9 mice for each group). (F) Time course of human leukocyte production in NSG recipients. Data are summarized from 2 trials of xenotransplants (n = 4 to 7 mice for each group). w, weeks.