Coregulation of TLR2 and TLR6 expression in MAMs. Lysates from CD14⁺ MAM obtained from BM aspirates at diagnosis were immunoblotted using a panel of antibodies against TLRs. Human primary BM-derived CD14⁺ cells and human primary peripheral blood (PB)-derived CD14⁺ cells served as controls. GAPDH provided a loading control. (A) TLR2/GAPDH. (B) TLR6/GAPDH. (C) TLR1/GAPDH. (D) TLR4/GAPDH. (E) TLR7/GAPDH. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.