Acute sickle nephropathy in a renal transplant biopsy specimen. (A) Hematoxylin and eosin stain of congested and large glomerulus. (B) Toluidine blue stain demonstrating sickle-shaped red blood cells and the glomerular capillaries. (C) Silver stain highlighting a congested peritubular capillary (black arrow). Panels A-C were taken on an Olympus BX50 microscope using an Olympus DP12 camera and Olympus image software (Melville, NY). Panels A and C were viewed with a ×40 objective at ×10 magnification (total magnification ×400); panel B was viewed with a ×20 objective ×10 magnification (total magnification ×200).