Sf3b1^+/− BM cells normally reconstitute hematopoiesis in vivo. (A) Contribution of donor cells to PB hematopoiesis. WT and Sf3b1^+/− BM cells (5 × 10⁶ cells, CD45.2⁺) were transplanted into lethally irradiated recipient (CD45.1⁺) mice. Chimerism of donor-derived CD45.2⁺ cells in the PB is shown as mean ± SD (n = 7). BMT, BM transplant. (B) Lineage contribution of WT and Sf3b1^+/− donor cells in PB. The proportion of each cell lineage in donor-derived CD45.2⁺ cells in the PB at 12 months posttransplantation. (C-D) Contribution of donor cells to BM hematopoiesis. Chimerism of donor-derived CD45.2⁺ cells in total BM cells and LSK cells is shown as mean ± SD (n = 7) in C. Absolute numbers of donor-derived CD45.2⁺ BM cells and LSK cells are shown as mean ± SD (n = 7) in D. N.S., not significant.