Comparative analysis of the resistance to anticancer regimens conferred by each of the 6 prosurvival Bcl-2 proteins in T-leukemic cells. J16 (Jurkat) T-ALL cells were retrovirally transduced to stably express untagged Bcl-2, Bcl-B, Bcl-w, Bcl-xL, Bfl-1, Mcl-1, or empty control vector (E.V.). All cell lines were treated with a dose range of the indicated conventional or experimental anticancer drugs, IR, or staurosporine. Cell death was assessed by PI uptake 48 hours after addition of the stimulus. E.V.-transduced cells were treated in absence or presence of the pan-caspase inhibitor Q-VD-OPH (20 µM) to demonstrate that cell death was apoptotic (E.V. Q). Data shown are mean values ± standard deviation (SD) derived from 3 independent experiments.