Clinical characteristics of patients with pancreatitis after treatment with BV, and autopsy specimen histology and quantitative immunohistochemistry with multispectral imaging (patient 1). (A) Autopsy specimen demonstrating fat necrosis of pancreas. (B) Autopsy specimen demonstrating coagulation necrosis of pancreas. (C) CD30 immunohistochemistry slide. Histogram: CD30 positivity in 27 (35%) of the 77 analyzed cells, with background optical density (OD) subtracted from all values. The bars signify specific CD30 staining. Insert: Standard light microscopic images of CD30 immunohistochemistry slide (left) and corresponding computer-generated image of pure 3,3′-diaminobenzidine signal (right), signifying CD30 expression in this biopsy. (D) Mouse immunoglobulin control slide. Histogram: The average OD values per cell are depicted. Here, a small subset of cells show OD values that exceed the range of 2 standard deviations from mean, an expected finding assuming a normal distribution of OD values. Insert: Standard light microscopic image of mouse immunoglobulin slide (left) and the corresponding computer-generated image of pure 3,3′-diaminobenzidine signal (right), showing nonspecific staining. Note: Data are expressed as average OD per cell. CD30 positivity is defined as any average OD per cell greater than nonspecific background OD (in this case, 0.021 plus 2 standard deviations from the mean, a value determined by measuring OD values in the mouse immunoglobulin control slide). cHL, classical Hodgkin Lymphoma; ALCL, anaplastic large cell lymphoma; CT, computed tomography. *Ultrasound showed only biliary sludge; **CTCAE 4.03 (evs.nci.nih.gov).