Mitochondrial uncoupling and ROS scavenging attenuates platelet activation. H₂O₂ production (A), ΔΨ (B), and activation levels (C) in untreated (control) healthy platelets or those exposed to hemoglobin (25 µmol/L) or oligomycin (0.4 µmol/L) in the absence (white bars) or presence of FCCP (0.35 µmol/L; gray bars), or mitoTEMPO (100 µmol/L; black bars). Data are presented as mean ± SEM (n > 4; P < .01).