SCD platelets show bioenergetic dysfunction. (A) Representative platelet bioenergetic profile from an SCD patient (red) and healthy control (black). (B) Rotenone-insensitive OCR of control and SCD patient platelets. Bars represent mean ± SEM. (C) Quantification of each OCR component of bioenergetic profile of platelets for an SCD patient and control after correction for nonmitochondrial OCR (n = 24 SCD; 19 controls). (D) Quantification of the percent of hyperpolarized mitochondria in platelets from SCD and control subjects (n = 16 each group). (E) Representative MitoSOX fluorescence images from control and SCD platelets and relative fluorescence intensity of MitoSOX labeling in control and SCD platelets (n = 18 each group). (F) Representative Amplex Red traces and quantification of H₂O₂ generation (normalized to 10⁶ cells) from control and SCD platelets (n = 14 each).