Validation of XF analysis for human platelets. (A) Typical bioenergetic profile of platelets from a healthy African-American subject. Arrows denote the addition of oligomycin (2.5 µmol/L), FCCP (0.7 µmol/L), and rotenone (10 µmol/L) to black trace. Gray trace is the same sample in the absence of modulators. All OCR rates are normalized to 10⁶ platelets. (B) Percent of activated platelets measured in 5 different samples before seeding in the XF analyzer and after measurement. (C) OCR of healthy platelets in the presence of rotenone. Arrows denote the addition of indomethacin (100 µmol/L) and allopurinol (100 µmol/L). (D) Basal (black squares) and maximal (open circles) OCR in increasing numbers of healthy human platelets. (E) Basal (black bars) and maximal (white bars) OCR in healthy platelets measured by XF analysis versus Clark electrode.