Figure 4
Figure 4. Normal LEC:BEC ratio in Clec1bfl/flPF4-Cre embryos and newborns. (A-C) Flow cytometric analysis of newborn mesenteric vessels (without the LNs, A), newborn intestine (B), and E18.5 lungs (C), single-cell suspensions from Clec1bfl/flPF4-Cre and Clec1bfl/fl control littermates, stained with CD45, Ter119, Gp38, and CD31. Percentages of CD31+Gp38− BEC and of CD31+Gp38+ LEC in the CD45−Ter119− stromal fraction are indicated in the dot plots of the second columns. Quantification of the ratios of LEC:BEC are shown on the charts.

Normal LEC:BEC ratio in Clec1bfl/flPF4-Cre embryos and newborns. (A-C) Flow cytometric analysis of newborn mesenteric vessels (without the LNs, A), newborn intestine (B), and E18.5 lungs (C), single-cell suspensions from Clec1bfl/flPF4-Cre and Clec1bfl/fl control littermates, stained with CD45, Ter119, Gp38, and CD31. Percentages of CD31+Gp38 BEC and of CD31+Gp38+ LEC in the CD45Ter119 stromal fraction are indicated in the dot plots of the second columns. Quantification of the ratios of LEC:BEC are shown on the charts.

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