Impaired LEC proliferation in Clec1b^−/− embryos. (A) Flow cytometric analysis of intestine single-cell suspensions from Clec1b^−/− and control littermates at E16.5 stained with CD45, Ter119, Gp38, CD31, and Ki67. The percentages of CD31⁺Gp38⁻ BEC and of CD31⁺Gp38⁺ LEC in the CD45⁻Ter119⁻ stromal fraction are indicated in the dot plots of the first column. The percentages of Ki67⁺ in the BEC and LEC compartment are shown in the dot plots of the second and third columns. (B-C) Quantifications of the ratio of LEC:BEC and of the ratio of Ki67⁺ in the BEC and LEC compartments are shown in the first, second, and third charts at E16.5 (B) and E18.5 (C) in the intestine. Unpaired Student t test, **P < .01, ***P < .005.