DNA methylation of hematopoietic transcription factors is reciprocal to changes in expression and motif activity. DNA methylation changes, transcription, and motif activity of PU.1, GATA2, and GFI1. (A) Graphs showing the β-values of DMSs annotated to respective genes. Each dot represents 1 DMS. (B) Box plots displaying the signals from expression microarray analysis for all 5 replicates (arbitrary units). (C) Bar chart demonstrating the relative motif activity for all CAGE samples (arbitrary units), ie, transcriptional activity of genes with promoters containing potential binding sites for PU.1, GATA2, or GFI1. Error bars indicate standard deviation. The GATA2 binding motif is shared with GATA1 and GATA3.