Changes in DNA methylation occur at distinct time points. (A) A total number of 10 156 DMSs were identified and subjected to hierarchical clustering for all cell populations from all 3 donors. C, CMP; G, GMP; P, PMC; N, PMN. DMSs were defined as CpGs with mean Δβ ≥ 0.17 between any 2 cell populations (eg, CMP vs GMP and CMP vs PMN). (B) All 10 156 DMSs were divided into (left) DMS up, (center) DMS down, or (right) DMS oscillating based on direction of methylation change. β-values were plotted between consecutive cell stages, ie, GMP vs CMP, PMC vs GMP, and PMN vs PMC. β-values of DMSs are shown as dots, and β-values for unchanged CpGs are shown as smooth scatter (blue and black dots).