Figure 6
Regulatory host cells are required for donor cell engraftment. Engraftment of 10 000 HSC following TLI/ATG was studied in 3 immune-deficient strains BALB/c Rag2γc−/−, B6 Rag2γc−/−, and B6.Jα18−/− and compared with WT recipients. (A) BLI used to monitor homing and expansion of B6.luc+ HSC revealed. In BALB/c Rag2γc−/− recipients, engraftment was slow and inconsistent, and 4 of 5 mice lost their graft (2 dead before week 8, 2 died between weeks 8 and 12). Images were compared with WT albino B6. (B) Compiled data on percent donor engraftment in the blood B cells (left graph), T cells (middle graph), CD11b+ (Mac1+) myeloid lineages (right graph) in WT B6 (white solid and white striped bars), and B6.Jα18−/− (black solid and black striped bars) recipients of congenic B6 or miAg-mismatched AKR/b (A/b) KTLS-HSC at 6 weeks post-HCT showing similar levels of mixed chimerism in WT and B6.Jα18−/− recipients. (C) Compiled levels of donor chimerism within immature CD4+CD8+ DP and more mature CD4+ and CD8+ SP thymic T-cell populations revealed higher proportions of donor cells in WT B6 (left) compared with B6.Jα18−/− (right) recipients of B6 and AKR/b-HSC grafts. Statistical significance was reached for differences between WT B6 and B6.Jα18−/− recipients of AKR/b but not B6.HSC grafts in DP (¥P = .09) and SP populations (‡: CD4+, P = .002; ◇: CD8+, P = .01). (D) Compiled levels of donor chimerism within LT-HSC in BM of legs and spine in WT B6 (left), B6.Jα18−/− (middle), and B6.Rag2γc−/− (right) recipients of congenic B6 vs miAg-mismatched AKR/b HSC grafts at 6 weeks post-TLI/ATG, and HCT showing lower proportions of donor LT-HSC in spines and legs of B6.Jα18−/− compared with WT recipients, and the lowest proportions in B6.Rag2γc−/− recipients, regardless of graft type. Statistical significance was reached in the following subsets: B6 donor: WT vs B6.Jα18−/− recipients (spine ¥: P = .01); WT vs B6.Rag2γc−/− recipient: (spine ◇: P = .035). AKR/b donor: WT vs B6.Rag2γc−/− recipients: (spine ‡: P = .02). N = 5 animals per experimental group.

Regulatory host cells are required for donor cell engraftment. Engraftment of 10 000 HSC following TLI/ATG was studied in 3 immune-deficient strains BALB/c Rag2γc−/−, B6 Rag2γc−/−, and B6.Jα18−/− and compared with WT recipients. (A) BLI used to monitor homing and expansion of B6.luc+ HSC revealed. In BALB/c Rag2γc−/− recipients, engraftment was slow and inconsistent, and 4 of 5 mice lost their graft (2 dead before week 8, 2 died between weeks 8 and 12). Images were compared with WT albino B6. (B) Compiled data on percent donor engraftment in the blood B cells (left graph), T cells (middle graph), CD11b+ (Mac1+) myeloid lineages (right graph) in WT B6 (white solid and white striped bars), and B6.Jα18−/− (black solid and black striped bars) recipients of congenic B6 or miAg-mismatched AKR/b (A/b) KTLS-HSC at 6 weeks post-HCT showing similar levels of mixed chimerism in WT and B6.Jα18−/− recipients. (C) Compiled levels of donor chimerism within immature CD4+CD8+ DP and more mature CD4+ and CD8+ SP thymic T-cell populations revealed higher proportions of donor cells in WT B6 (left) compared with B6.Jα18−/− (right) recipients of B6 and AKR/b-HSC grafts. Statistical significance was reached for differences between WT B6 and B6.Jα18−/− recipients of AKR/b but not B6.HSC grafts in DP (¥P = .09) and SP populations (‡: CD4+, P = .002; ◇: CD8+, P = .01). (D) Compiled levels of donor chimerism within LT-HSC in BM of legs and spine in WT B6 (left), B6.Jα18−/− (middle), and B6.Rag2γc−/− (right) recipients of congenic B6 vs miAg-mismatched AKR/b HSC grafts at 6 weeks post-TLI/ATG, and HCT showing lower proportions of donor LT-HSC in spines and legs of B6.Jα18−/− compared with WT recipients, and the lowest proportions in B6.Rag2γc−/− recipients, regardless of graft type. Statistical significance was reached in the following subsets: B6 donor: WT vs B6.Jα18−/− recipients (spine ¥: P = .01); WT vs B6.Rag2γc−/− recipient: (spine ◇: P = .035). AKR/b donor: WT vs B6.Rag2γc−/− recipients: (spine ‡: P = .02). N = 5 animals per experimental group.

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