Figure 1
Figure 1. T-cell isolation and expansion. (A) Sorting strategy for CD4+CD25highCD45RA+ (pRA+Treg), CD4+CD25highCD45RA– (pRA–Treg), CD4+CD25–CD45RA+ (pRA+Tconv) and CD4+CD25–CD45RA– (pRA–Tconv) from human peripheral blood mononuclear cells (PBMCs) as described in “Methods”. (B) Cells were reanalyzed after sorting on the FACSAria.

T-cell isolation and expansion. (A) Sorting strategy for CD4+CD25highCD45RA+ (pRA+Treg), CD4+CD25highCD45RA– (pRA–Treg), CD4+CD25–CD45RA+ (pRA+Tconv) and CD4+CD25–CD45RA– (pRA–Tconv) from human peripheral blood mononuclear cells (PBMCs) as described in “Methods”. (B) Cells were reanalyzed after sorting on the FACSAria.

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