Expression patterns of epigenetic factors validated by qRT-PCR. The qRT-PCR transcript levels were normalized to actin gene expression, and the relative fold enrichment was calculated over the cell type with the lowest expression for each gene (y-axis). (A) The relative fold enrichment of transcript levels of HELLS, ACTL6A, and MLL was determined by qRT-PCR. B cells are depicted as B, T cells as T, CD34⁺ progenitors as P, granulocytes as G, and monocytes as M. (B) Differential expression patterns of 6 epigenetic factors in different cell types validated by qRT-PCR (gray bars) and compared with the CAGE average TPM values (orange bars). The expression of these genes wa validated using total RNA obtained from 3 individual healthy donors separate from those used for CAGE analysis.