Figure 4
Figure 4. Loss of Bmf enhances the B cell-restricted lymphadenopathy caused by loss of Bim. (A-H) Spleens were harvested from 7- to 9-week-old mice of the indicated genotypes and single cell suspensions were counted to assess total leukocyte cellularity. Cell suspensions were stained using different combinations or fluorochrome-labeled antibodies for identification of total splenic T and B cells, as well as T1, T2, MZ, and FO B cells and PC/PBs. Data are presented as means ± SEM of ≥4 independent experiments and 4 to 8 animals per genotype. Significant differences by unpaired Student t test (P < .05) are indicated *between WT and Bmf−/−, **between WT and Bim−/−, #between WT and DKO, and §between Bim−/− and Bim/Bmf DKO mice.

Loss of Bmf enhances the B cell-restricted lymphadenopathy caused by loss of Bim. (A-H) Spleens were harvested from 7- to 9-week-old mice of the indicated genotypes and single cell suspensions were counted to assess total leukocyte cellularity. Cell suspensions were stained using different combinations or fluorochrome-labeled antibodies for identification of total splenic T and B cells, as well as T1, T2, MZ, and FO B cells and PC/PBs. Data are presented as means ± SEM of ≥4 independent experiments and 4 to 8 animals per genotype. Significant differences by unpaired Student t test (P < .05) are indicated *between WT and Bmf−/−, **between WT and Bim−/−, #between WT and DKO, and §between Bim−/− and Bim/Bmf DKO mice.

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