CD16xCD33 BiKE consistently enhances NK cell function at all disease stages. The percent of CD56⁺/CD3⁻ MDS-NK cells (A), total CD33⁺ cells (B), and MDS-MDSC (C) of the all-cell fraction was stratified according to their respective FAB classification and IPSS karyotype groups. (D-E) CD16xCD33 BiKE-induced MDS-NK cell functions (degranulation [CD107a], intracellular TNF-α and IFN-γ production) in the absence of HL-60 targets (D) and in the presence of HL-60 targets (E) were stratified according to their respective FAB classification and IPSS karyotype groups. The y-axes of graphs in panels D and E represent the percent of NK cells positive for each function listed in the graph legend.