Integrin homeostasis in Srf WT and KO neutrophils. (A) Expression of integrin CD11b, CD18, and CD11a on the cell surface of Srf WT and KO neutrophils by flow cytometry. Srf WT and KO neutrophils were incubated with fluorescently labeled ICAM-1 (B) and stimulated with fMLP for 0, 2, and 5 minutes; (C) CD11b surface expression was determined at the same time. (Representative experiment of 3 independent experiments performed in triplicate; **P < .005, ***P < .0005.) (D) Srf WT and KO neutrophils were stimulated with vehicle or fMLP for 15 minutes at 37°C and lysates probed for Itgam, Erk, and P-Erk, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control. Lysates are from 1 experiment; vertical lines have been inserted to indicate a repositioned gel lane. Neg ctrl, negative control.