RNAs associated with age. (A) Multicomponent heatmap of RNAs DE by age with each of 154 subjects represented as a column. The upper heatmap stripe indicates age. The second heatmap stripe indicates composite mitochondrial mRNAs (Mito). The third and fourth heatmaps indicate mRNAs positively and negatively associated with age, respectively. The fifth and sixth heatmaps show miRNA positively and negatively associated with age, respectively. Odds ratios of (B) enriched GO categories among mRNAs DE by age and (C) putative targets of miRNAs DE by age. (D) A network of miRNAs-mRNAs DE by age. For the network of RNAs DE by age, we required that (1) both the miRNA and mRNA were DE by age; (2) the miRNA was predicted to target the mRNA by ≥3 different target prediction algorithms contained in mirDIP; and (3) the Spearman correlation coefficient between miRNA abundance and mRNA abundance levels was less than −0.2 (supplemental Table 6).