Figure 6
Figure 6. Delayed aggregation in platelets expressing talin1(W359A). (A) Aggregation response of washed Tln1wt/flCre+ (black line), Tln1W359A/flCre+ (gray line), Tln1L325R/flCre+ (gray dashed line), and Tln1fl/flCre+ platelets (black dashed line) stimulated with 5 μg/mL (LD) collagen, 25 μg/mL (HD) collagen, 1 μM U46619, 200 ng/mL convulxin, 200 μM (LD), or 600 μM (HD) Par4-AP. (B) Calcium mobilization in platelets labeled with the calcium-sensitive dye Fluo-4 and stimulated with Par4-AP in the presence of 1 mM Ca2+. (C) Time course of Rap1 activation in platelets stimulated with Par4-AP. The bottom panel shows total Rap1 as a loading control. Results are representative of 3 independent experiments.

Delayed aggregation in platelets expressing talin1(W359A). (A) Aggregation response of washed Tln1wt/flCre+ (black line), Tln1W359A/flCre+ (gray line), Tln1L325R/flCre+ (gray dashed line), and Tln1fl/flCre+ platelets (black dashed line) stimulated with 5 μg/mL (LD) collagen, 25 μg/mL (HD) collagen, 1 μM U46619, 200 ng/mL convulxin, 200 μM (LD), or 600 μM (HD) Par4-AP. (B) Calcium mobilization in platelets labeled with the calcium-sensitive dye Fluo-4 and stimulated with Par4-AP in the presence of 1 mM Ca2+. (C) Time course of Rap1 activation in platelets stimulated with Par4-AP. The bottom panel shows total Rap1 as a loading control. Results are representative of 3 independent experiments.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal