Runx proteins and Cbfβ restrict GMP-skewed differentiation of MPPs. (A) Microarray analysis of gene expression in LSK cells and GMPs from Vav1-iCre⁺Cbfb^F/+ (Het) and Vav1-iCre⁺Cbfb^F/F (CKO) mice presented as scattered plots. Signals with higher than threefold differences between Het and CKO samples are shown. (B) Expression of representative “GMP-specific” and “MDP-specific” genes in LSK cells is shown as a heat map. Expression of each gene is shown relative to control (Het) LSK cells. (C) qRT-PCR analysis of Cbfb, Irf8, Sfpi1 (PU.1), Cebpa (C/EBPα), and Csf1r (MCSF-R) in GMPs. Expression levels were normalized against the levels of 18S ribosomal RNA and shown as relative to Het control. Values show mean and standard deviation (n = 4); *P < .05. (D) In vitro DC differentiation from Cbfb-CKO BM cells with retroviral overexpression of Cbfβ (2 isoforms, n = 3), Irf8 (n = 3), and Flt3 (n = 2). CD11c⁺MHC-II⁺ and CD11c^–MHC-II⁺ cells are gated with rectangles, and percentages from a representative experiment are shown.