Cbfb is required for the development of DCs. (A) Splenocytes from 6- to 8-week-old Vav1-iCre⁺Cbfb^F/+ (Het) and Vav1-iCre⁺Cbfb^F/F (CKO) mice were analyzed for CD11c, MHC-II, and B220 expression. CD11c⁺MHC-II⁺ cDCs in B220^– gated splenocytes are shown with rectangle gates and percentages. Statistical analysis is shown in panel E as mean and standard deviation (n = 8). (B) Bst2 and SiglecH expression in total splenocytes from Vav1-iCre⁺Cbfb^F/+ and Vav1-iCre⁺Cbfb^F/F mice. Bst2⁺SiglecH⁺ pDCs are shown with rectangle gates and percentages, and statistical analysis is shown as mean and standard deviation in panel F (n = 4). (C) Mononuclear cells from lung, kidney, and SI in 6- to 8-week-old Vav1-iCre⁺Cbfb^F/+ and Vav1-iCre⁺Cbfb^F/F mice were analyzed for expression of CD45, CD11c, and MHC-II. CD11c⁺MHC-II⁺ cDCs in CD45⁺ gated cells are shown with rectangle gates. Mean percentages and standard deviation of total CD45⁺ cells from 4 mice are shown (n = 4). (D) Statistical analysis of total splenocyte numbers from Vav1-iCre⁺Cbfb^F/+ and Vav1-iCre⁺Cbfb^F/F mice shown as mean and standard deviation (n = 8). (G-H) Total BM cells were cultured in the presence of GM-CSF (G, n = 2) or Flt3L (H, n = 4) in vitro, and DC differentiation was examined by expression of CD11c, MHC-II, Ly6G, CD45RA, and SiglecH using flow cytometry. Statistical difference was assessed with the unpaired 2-tailed Student t test. *P < .05; **P < .01. SI, small intestine.