FLT3-ITD expression induced evident p-stat5, p-erk, and p-akt, and colocalized with mpo. (A) Western blotting comparing the FLT3, p-FLT3, stat5, p-stat5, erk1/2, p-erk1/2, akt, and p-akt in uninjected, FLT3-WT, and FLT3-ITD embryos at 36 hpf. (B-E) Double-color WISH showing the mpo and egfp expression in uninjected control (B-C) and FLT3-ITD overexpressing (D-E) embryos at 36 hpf. The boxed area indicates the coexpression of mpo and egfp in the yolk sac. Scale bars represent 500 μm.