flt3 inhibitor recapitulated the effect of flt3 MO knockdown. AC220 (2.5 μmol/L) was used to treat the embryos from 6 hpf to defined developmental stage. Equal volume of DMSO treatment was used as control. (A-B) WISH comparing the l-plastin expression in the anterior yolk sac region between DMSO control (A) and AC220 (B) embryos at 19 hpf. (C) Comparison of l-plastin expression in DMSO control and AC220 embryos at 19 hpf. (D-I) WISH comparing the expression of c-myb (D-E), flt4 (F-G), and efnb2α (H-I) between DMSO control (D,F,H) and AC220 (E,G,I) embryos at 32 hpf. The arrowheads in panel D indicated c-myb expression along the ventral wall of DA. (J-M) WISH showing the expression of c-myb (J-K) and rag1 (L-M) expression in DMSO control (J,L) and AC220 (K,M) embryos at 96 hpf. The arrow and arrowheads in panel J indicated c-myb expression at the thymus and CHT. The circled region (L-M) indicates the rag1 expression in the thymus. Scale bars represent 500 μm.