Platelet PDI is important for thrombus formation on collagen-coated surfaces under arteriolar shear. Blood drawn from WT and PDI CKO mice was perfused over collagen-coated surfaces at a wall shear rate of 1000 s−1 for 1 minute. In some experiments, wtPDI or dmPDI was added to blood drawn from PDI CKO mice and then blood was perfused through the chamber. Adherent thrombi were stained with rhodamine-conjugated phalloidin and analyzed as described in “Materials and methods.” (A) Representative images. Bar = 10 μm. (B,C) Surface coverage and thrombus volume were measured and presented as mean ± SD (n = 4). ***P < .001 vs WT platelets after ANOVA and Dunnett test; ##P < .01; and ###P < .001 vs PDI-null platelets treated with wtPDI after Student t test.
