The therapeutic application of MKEY decreases the severity of VILI. WT mice were treated with 3 mg/kg MKEY or scrambled control peptide 15, 60, or 90 minutes after the induction of mechanical ventilation with sham or VILI settings. (A) The paO₂/FiO₂ ratio, (B) neutrophil recruitment into the alveoli, and (C) protein concentration in the BAL were analyzed after 2 hours (n = 4). (D) The formation of circulating platelet-neutrophil aggregates was measured by flow cytometry 30 minutes after induction of sham or VILI ventilation (n = 4). (E) Circulating NET structures were quantified by MPO-DNA-ELISA (n = 4). *P < .05.