IFN-γ–stimulated genes in FBM and ABM classical bone marrow monocytes. (A) Cells were stimulated with 40 ng/mL IFN-γ for 4 hours and the sorted to >99% purity for whole-genome gene microarray analysis. Panels A to C depict a representative subset of the differentially expressed genes. (A) A subset of genes is not differentially expressed at baseline between ABM and FBM but is significantly more highly expressed in FBM than ABM after stimulation with IFN-γ (based on relative expression and an FDR ≤ 5%). Relative expression is the log₂ representation of gene expression compared with median normalized array values. All genes marked with an asterisk (*) have bioinformatically identified putative STAT1 binding sites in their promoters; all those marked with a dagger (†) have bioinformatically identified putative STAT5 binding site in their promoter. (B) A subset of genes is not differentially expressed at baseline between ABM and FBM but is significantly more highly expressed in ABM than FBM after stimulation with IFN-γ (based on relative expression and FDR ≤ 5%). (C) A subset of genes that is not differentially expressed at baseline between ABM and FBM and is not significantly differentially expressed between ABM and FBM and are induced in both subsets after stimulation with IFN-γ (based on relative expression and an FDR ≤ 5%). (D) Pie chart of putative STAT1 and STAT5 binding sites in the promoters of significantly differentially expressed genes. NA indicates that there is no bioinformatic information (on any transcription factor) available on the gene of interest.