ERG/Gata1s FL cells present an early erythroid expression profile. (A) GSEA using gene expression of human erythroid cells at different stages of differentiation38 shows enrichment of Wt/Gata1s and ERG/GATA1s E14.5 FL gene signature (GSE46481) in early erythroid cells. (B) Heat map showing the top 20 core enrichment genes from the GSEA presented in panel A, right. (C) Expression level of Gata2 and Myb genes as obtained from the mouse gene 1.0 ST chip array and confirmed by real-time PCR on at least 2 additional samples for each genotype. (D) Representative western blot from G1ME cells transduced with MigR1, MIGR1-GATA1, and MIGR1-GATA1s using the indicated antibodies. (E) Average difference in GATA2 expression from 3 independent G1ME expreriments presented in panel D. (F) An average repression of GATA2 mRNA by real-time PCR in E14.5 GATA1s KI FL cells infected with MIGR1-GATA1and MIGR1-GATA1s relative to MigR1-infected cells. Relative GATA1 and GATA1s RNA expression levels are indicated below (n = 3).
