FA deficiency affects Foxp3 transcriptional activity. (A) Relative expression levels of Foxp3 signature genes in WT and Fanca^−/− (upper) or Fancd2^−/− (lower) donor T cells. RNA extracted from H-2^b+CD4⁺ cells isolated from recipients transplanted with Treg cells from WT, Fanca^−/−, or Fancd2^−/− mice was used for real-time PCR analysis using primers specific for the indicated Foxp3 target genes. Samples were normalized to the level of GAPDH mRNA. (B) Change in expression levels of Foxp3 target genes in FA infiltrated donor T cells (H-2^b+CD4⁺). RNA extracted from H-2^b+CD4⁺ cells of the recipients described in (A) was used for real-time PCR analysis using primers specific for the indicated Foxp3 target genes. Each group includes 6 mice.