PKM2 regulates ALCL tumorigenesis. Methylcellulose colony formation assay using (A) DEL cells treated with 10 and 30 μM NCGC-527 and (B) DEL cells stably expressing Flag-PKM2 WT and Y105F. Samples analyzed in triplicate, with a representative image shown below each bar. Data are mean ± SD. (C) Tumor volumes of DEL xenograft tumors treated with vehicle (0.5% methylcellulose + 0.1% Tween 80) or TEPP-46 (50 mg/kg) twice a day by oral dose from day of tumor implantation (mean ± SEM) (n = 7). (D) Representative images of tumors at day 14. (E) Tumor volumes of xenografted DEL cells stably expressing WT or Y105F-PKM2 (mean ± SEM; n = 5). (F) Scatterplot of day 10 tumor volumes. Data are mean ± 95% confidence interval; *P < .05, **P < .01.