PKM2 regulates metabolic switch and proliferation. (A) PKM2 activity assay using SU-DHL-1 treated with 10 μM NCGC-527 for 24 hours. (B) Lactate and ATP assays using conditioned media and cell lysates (respectively) on Karpas299, DEL, and Jurkat cells following 6-hour treatment with 30 μM NCGC-527. (C) Lactate and ATP assays of DEL cells stably expressing Flag-PKM2 WT of Flag-PKM2 Y105F. (D) Cellular proliferation measured by serial counting with Trypan blue stain of DEL cells treated with indicated concentrations of NCGC-527. Cells were maintained in either normoxic or hypoxic (3% O2) conditions. (E) Viability data from D showing the percentage live cells under the indicated conditions. (F) Cell proliferation of DEL cells treated with NCGC-527, oligomycin, or both. (G) Cell proliferation of DEL cells stably expressing Flag-PKM2 and treated with DMSO or 500 nM oligomycin for 72 hours. Counts were normalized to each control (DMSO) condition. Data are mean ± SD; *P < .05, **P < .01, not significant (NS).
