Partial karyotypes of 2 patients demonstrating initial JT1q12 to RC MYC and a second sequential JT1q12 to different RCs. FISH probes 1q12 (red) and 1q21 (green) are shown on the left, SKY image-classified colors for chromosomes 8 (orange) and 1q (yellow) are in the middle, and the FISH probe for MYC (red) is on the right. (A) Patient 1 shows a single copy of JT1q12 translocated to 8q24 (left bracket), and in the same clone, a second translocation of the JT1q12 has translocated an extra copy of MYC to 3q (right bracket). (B) Patient 31 demonstrates a JT1q12 on chromosome 8q24 as the sole 1q aberration in the stemline. (C) In a second specimen analyzed 3 months later, a second translocation of the JT1q12 translocated an additional copy of MYC to 7q23. The sequential translocations of JT1q12 in both cases resulted in a CN increase for both MYC and 1q21 and deletions in the second RCs. Note: In all figures, FISH hybridizations are shown in inverse 4′,6 diamidino-2-phenylindole to delineate chromosome similarities to the G-banding patterns used in routine studies.