Regulation of IL-12 production by mDC1. (A) IL-12p70 production by peripheral blood DC subsets after a 24-hour culture in medium alone (−) or in the presence of the indicated TLR agonists. (B) Effects of IFN-γ, (C) CD40L, and (D) IL-4 on IL12p70 produced by mDC1. (E) IL-12 production by mDC1 (left column) and mDC2 (right column) in response to polyI:C, R848, and IFN-γ. Values show the mean of 16 donors in 8 different experiments. (F) IL-12p70 production by DC subsets in tonsils in response to the indicated stimuli. Results from 7 donors in 5 experiments are shown. nd, not determined. *P < .05; **P < .005.