Frequency and proliferation of human DC subsets in bone marrow, peripheral blood, and tonsils. (A) Gating strategy for human DC subsets in peripheral blood and tonsils. (B) Frequency of mDC1 (dark gray boxes), mDC2 (light gray boxes), and pDC (white boxes) in total mononuclear cells isolated from bone marrow, blood, and tonsils. (C) Mean fluorescence intensity (MFI) of the Ki67 proliferation marker in DC subsets from peripheral blood of healthy donors and tonsils. (D) Ki67 expression of DC subsets from bone marrow and peripheral blood of patients that were undergoing hip arthroplasty. Shown are results of 6 donors in at least 2 experiments. *P < .05; **P < .005; ***P < .0005.