Figure 1
Figure 1. HO-1 and globin expression is increased in Ter119+ cells from mouse bone marrow and during the differentiation of FL cells. (A-B) Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) of mouse bone marrow cells, sorted by their expression of Ter119, showing globin and HO-1 mRNA. (C) Western blot of HO-1 and globin protein of Ter119+/− cells. (D) qRT-PCR showing HO-1 mRNA levels in FL cells in a noninduced (0 hours) state or after their differentiation at the indicated time points. SA (0.4 µM), was added 24 hours before harvest. (E) Representative western blot of HO-1 and globin protein of FL cells in a noninduced (0 hours) state or after their differentiation at the indicated time points. (F) Densitometric analysis of HO-1 protein levels normalized to β-actin. Error bars of graphs represent standard deviation of 3 independent experiments (n = 3). *P < .05; **P < .001.

HO-1 and globin expression is increased in Ter119+ cells from mouse bone marrow and during the differentiation of FL cells. (A-B) Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) of mouse bone marrow cells, sorted by their expression of Ter119, showing globin and HO-1 mRNA. (C) Western blot of HO-1 and globin protein of Ter119+/− cells. (D) qRT-PCR showing HO-1 mRNA levels in FL cells in a noninduced (0 hours) state or after their differentiation at the indicated time points. SA (0.4 µM), was added 24 hours before harvest. (E) Representative western blot of HO-1 and globin protein of FL cells in a noninduced (0 hours) state or after their differentiation at the indicated time points. (F) Densitometric analysis of HO-1 protein levels normalized to β-actin. Error bars of graphs represent standard deviation of 3 independent experiments (n = 3). *P < .05; **P < .001.

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