Major Peak drives T-cell specific expression of luciferase in stable transfection assays. (A) The PR3 sequence of Bcl11b promoter region and Major Peak were cloned into the pGL3-Basic vector. (B) pGL3-Basic, pGL3-Control (SV40-PR; SV40-En), PR3, PR3-MP constructs were stably transfected into Raw264.7, NFS25, P2C2, and EL4 cells with pTracer-Renilla luciferase construct. The firefly luciferase activities were normalized to Renilla luciferase activities. The Normalized Expression was calculated by designation of the geomean of pGL3-Basic as 1 unit. •, relative firefly luciferase activities normalized by Renilla luciferase activities. -, geomeans of the data points in the same sample. Data shown are from one experiment representative of 3.