Survival and bleeding of afibrinogenemic zebrafish. A generation of adult zebrafish from mating of heterozygous animals from each mutant line was genotyped for exon 2 of the fga gene aged 4 to 5 months. The percentage of fish for each fga genotype, wild-type (+/+), heterozygous (+/−), and homozygous mutant fish (−/−) is represented in A. The total number of live animals genotyped for each line in this generation is shown to the right of each bar. Compared with an expected Mendelian ratio, homozygous mutant fish (−/−) are underrepresented and wild-type (+/+) fish overrepresented in animals from heterozygous mutation 1 and 2 crosses. In B-D, unprompted bleeding events from mutation 1 and 2 fish are represented. Black and white arrows highlight sites of bleeding. B shows a cephalic and ocular bleed with right side (left) and dorsal views (right). C shows a ventral hemorrhage with left side (left) and ventral views (right), and D shows a dorsal view of a cephalic bleed (left) with a nonbleeding fish for comparison (right). Scale bars are given in each image. Images were acquired using a Leica MZ16FA stereomicroscope equipped with a DFC420 digital camera and the Leica Application Suite software V4.2. A ×1.0 Planapochromatic objective was used. The zoom magnification was between 0.71 and 1.48 with a visual magnification between 7.1 and 14.8. The numerical aperture was 0.020 (B-C) or 0.040 (D). Color was set to a saturation setting of 1.7 to 2.0 and γ at 0.99. Images were taken at room temperature.