![Hepatic hepcidin and Id-1 mRNA levels in mice 72 hours after injection with Ad.IL-6 or Ad.GFP. (A) Hepatic hepcidin mRNA levels were determined by quantitative RT-PCR in Alk2fl/fl and Alk2fl/fl; Alb-Cre mice (1-way ANOVA, P = .009; #P = .0008: Alk2fl/fl mice injected with Ad.GFP [n = 3] vs Alk2fl/fl mice injected with Ad.IL-6 [n = 5]; †P = .01: Alk2fl/fl; Alb-Cre mice injected with Ad.GFP [n = 4] vs Alk2fl/fl; Alb-Cre mice injected with Ad.IL-6 [n = 7]). (B) Hepatic hepcidin mRNA levels in Alk3fl/fl and Alk3fl/fl; Alb-Cre mice (1-way ANOVA, P = .0005; *P = .03: Alk3fl/fl mice injected with Ad.GFP [n = 4] vs Alk3fl/fl; Alb-Cre mice injected with Ad.GFP [n = 6]; #P = .02: Alk3fl/fl mice injected with Ad.GFP vs Alk3fl/fl mice injected with Ad.IL-6 [n = 5]). As highlighted in the inset, hepatic hepcidin mRNA levels in Alk3fl/fl; Alb-Cre mice injected with Ad.GFP did not differ from those in Alk3fl/fl; Alb-Cre mice injected with Ad.IL-6. (C) Hepatic Id-1 mRNA levels in Alk2fl/fl and Alk2fl/fl; Alb-Cre mice injected with Ad.GFP or Ad.IL-6 (1-way ANOVA, P = .01; #P < .04: Alk2fl/fl mice injected with Ad.GFP [n = 3] vs Alk2fl/fl mice injected with Ad.IL-6 [n = 5]; †P = .0045: Alk2fl/fl; Alb-Cre mice injected with Ad.GFP [n = 4] vs Alk2fl/fl; Alb-Cre mice injected with Ad.IL-6 [n = 5]). (D) Hepatic Id-1 mRNA levels in Alk3fl/fl and Alk3fl/fl; Alb-Cre mice injected with Ad.GFP or Ad.IL-6 (1-way ANOVA, P = .0023; *P = .01: Alk3fl/fl injected with Ad.GFP vs Alk3fl/fl; Alb-Cre mice injected with Ad.GFP [n = 5]; #P = .024: Alk3fl/fl mice injected with Ad.GFP vs Alk3fl/fl mice injected with Ad.IL-6 [n = 5]). Hepatic Id-1 mRNA levels in Alk3fl/fl; Alb-Cre mice injected with Ad.GFP did not differ from those in Alk3fl/fl; Alb-Cre mice injected with Ad.IL-6.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/123/14/10.1182_blood-2013-02-480095/4/2261f2.jpeg?Expires=1721973966&Signature=uc56zxQ~-8q-PV3MxyJCpARVVWJUYDeCEChoacD5q6XYdI-WsOYv5R2~XG6XNmFh6DPwGuu1YJyy~Y7-2faDNcjvuEGQSKdTFdf2u8ekCkOb5qgdx3dyg0ynMtIjJHEe~MGoey8N9bDDGdlE0-6C3oCi7VUkJCzrIh84whipoCTzRGcKT~-ATRx-Mr0Hxpx1vmlHz3wLhCSbphEYf~aU18V3eyUfi5mhpumAH7FDv9vgjOW9asiIHSpg0sa5vSw~eRgNFz3BNxYvTXkJeN96DFXb0ueJxjCYxgfMsPgvL1m55SDN4XjSevAbPtZt3rM1jNkI4SzBDJ6r50D3aUCIQg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Hepatic hepcidin and Id-1 mRNA levels in mice 72 hours after injection with Ad.IL-6 or Ad.GFP. (A) Hepatic hepcidin mRNA levels were determined by quantitative RT-PCR in Alk2fl/fl and Alk2fl/fl; Alb-Cre mice (1-way ANOVA, P = .009; #P = .0008: Alk2fl/fl mice injected with Ad.GFP [n = 3] vs Alk2fl/fl mice injected with Ad.IL-6 [n = 5]; †P = .01: Alk2fl/fl; Alb-Cre mice injected with Ad.GFP [n = 4] vs Alk2fl/fl; Alb-Cre mice injected with Ad.IL-6 [n = 7]). (B) Hepatic hepcidin mRNA levels in Alk3fl/fl and Alk3fl/fl; Alb-Cre mice (1-way ANOVA, P = .0005; *P = .03: Alk3fl/fl mice injected with Ad.GFP [n = 4] vs Alk3fl/fl; Alb-Cre mice injected with Ad.GFP [n = 6]; #P = .02: Alk3fl/fl mice injected with Ad.GFP vs Alk3fl/fl mice injected with Ad.IL-6 [n = 5]). As highlighted in the inset, hepatic hepcidin mRNA levels in Alk3fl/fl; Alb-Cre mice injected with Ad.GFP did not differ from those in Alk3fl/fl; Alb-Cre mice injected with Ad.IL-6. (C) Hepatic Id-1 mRNA levels in Alk2fl/fl and Alk2fl/fl; Alb-Cre mice injected with Ad.GFP or Ad.IL-6 (1-way ANOVA, P = .01; #P < .04: Alk2fl/fl mice injected with Ad.GFP [n = 3] vs Alk2fl/fl mice injected with Ad.IL-6 [n = 5]; †P = .0045: Alk2fl/fl; Alb-Cre mice injected with Ad.GFP [n = 4] vs Alk2fl/fl; Alb-Cre mice injected with Ad.IL-6 [n = 5]). (D) Hepatic Id-1 mRNA levels in Alk3fl/fl and Alk3fl/fl; Alb-Cre mice injected with Ad.GFP or Ad.IL-6 (1-way ANOVA, P = .0023; *P = .01: Alk3fl/fl injected with Ad.GFP vs Alk3fl/fl; Alb-Cre mice injected with Ad.GFP [n = 5]; #P = .024: Alk3fl/fl mice injected with Ad.GFP vs Alk3fl/fl mice injected with Ad.IL-6 [n = 5]). Hepatic Id-1 mRNA levels in Alk3fl/fl; Alb-Cre mice injected with Ad.GFP did not differ from those in Alk3fl/fl; Alb-Cre mice injected with Ad.IL-6.
