![Serum iron concentrations and transferrin saturation in mice injected with Ad.GFP or Ad.IL-6. (A) Serum iron concentrations were measured in Alk2fl/fl and Alk2fl/fl; Alb-Cre mice 72 hours after intravenous injection of 1 × 1011 virus particles of an adenovirus specifying green fluorescent protein (Ad.GFP) or an adenovirus specifying interleukin 6 (Ad.IL-6). The presence of the Cre recombinase transgene is indicated below the x-axis as Cre “+” (1-way ANOVA, P = .0018. *P = .006: Alk2fl/fl mice injected with Ad.GFP [n = 3] vs Alk2fl/fl; Alb-Cre mice injected with Ad.GFP [n = 5]; #P = .02: Alk2fl/fl mice injected with Ad.GFP vs Alk2fl/fl mice injected with Ad.IL-6 [n = 5]; †P = .03: Alk2fl/fl; Alb-Cre mice injected with Ad.GFP vs Alk2fl/fl; Alb-Cre mice injected with Ad.IL-6 [n = 7]). (B) Transferrin saturations in Alk2fl/fl and Alk2fl/fl; Alb-Cre mice 72 hours after intravenous injection of Ad.GFP or Ad.IL-6 (1-way ANOVA, P = .001; *P = .007: Alk2fl/fl mice injected with Ad.GFP vs Alk2fl/fl; Alb-Cre mice injected with Ad.GFP; #P = .01: Alk2fl/fl injected with Ad.GFP vs Alk2fl/fl injected with Ad.IL-6; †P = .001: Alk2fl/fl; Alb-Cre mice injected with Ad.GFP vs Alk2fl/fl; Alb-Cre mice injected with Ad.IL-6). (C) Serum iron concentrations were measured in Alk3fl/fl and Alk3fl/fl; Alb-Cre mice injected with Ad.GFP or Ad.IL-6 (1-way ANOVA, P = .0016; *P = .0004: Alk3fl/fl mice injected with Ad.GFP [n = 4] vs Alk3fl/fl; Alb-Cre mice injected with Ad.GFP [n = 6]; #P = .0445: Alk3fl/fl mice injected with Ad.GFP vs Alk3fl/fl mice injected with Ad.IL-6 [n = 4]). Serum iron concentrations in Alk3fl/fl; Alb-Cre mice injected with Ad.GFP did not differ from those in Alk3fl/fl; Alb-Cre mice injected with Ad.IL-6 (P = .6). (D) Transferrin saturations in Alk3fl/fl and Alk3fl/fl; Alb-Cre mice 72 hours after injection with Ad.GFP or Ad.IL-6 (1-way ANOVA, P = .0004; *P = .0001: Alk3fl/fl mice injected with Ad.GFP vs Alk3fl/fl; Alb-Cre mice injected with Ad.GFP; #P = .004: Alk3fl/fl mice injected with Ad.GFP vs Alk3fl/fl mice injected with Ad.IL-6; †P = .003: Alk3fl/fl; Alb-Cre mice injected with Ad.GFP vs Alk3fl/fl; Alb-Cre mice injected with Ad.IL-6).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/123/14/10.1182_blood-2013-02-480095/4/2261f1.jpeg?Expires=1721973078&Signature=kV9PPCfK-8Z04gk3NM80Ro8AFG5QPHP21rYK3p~YMRQQ4rwq94NlyBgg276JjL6DoTU9LPxrrcBAl7iTZBmAcGqabPAvboC2plY83OgkhM3~FV2kfhH-8gwVQJ0Ktok2v3RvzId5YsVZACMS77V9SdIydltIIz9j8jrS-tBlNOlWUatvaXwJCj9mYkAa7J7yTgBCTxnvm-gHoVpZ7ea4WMVBprqGbS3rrIk-qH10T8Cd2FVMUyDz2NOjYIfFytU4zcO9TJb2vfU8r1svjXyi3mc~cVqpXqaVGHf~iwFF0i8r5afd6jVfjo7MqyXhzjXtpPtTMohdiqFS~QO~alIFdw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Serum iron concentrations and transferrin saturation in mice injected with Ad.GFP or Ad.IL-6. (A) Serum iron concentrations were measured in Alk2fl/fl and Alk2fl/fl; Alb-Cre mice 72 hours after intravenous injection of 1 × 1011 virus particles of an adenovirus specifying green fluorescent protein (Ad.GFP) or an adenovirus specifying interleukin 6 (Ad.IL-6). The presence of the Cre recombinase transgene is indicated below the x-axis as Cre “+” (1-way ANOVA, P = .0018. *P = .006: Alk2fl/fl mice injected with Ad.GFP [n = 3] vs Alk2fl/fl; Alb-Cre mice injected with Ad.GFP [n = 5]; #P = .02: Alk2fl/fl mice injected with Ad.GFP vs Alk2fl/fl mice injected with Ad.IL-6 [n = 5]; †P = .03: Alk2fl/fl; Alb-Cre mice injected with Ad.GFP vs Alk2fl/fl; Alb-Cre mice injected with Ad.IL-6 [n = 7]). (B) Transferrin saturations in Alk2fl/fl and Alk2fl/fl; Alb-Cre mice 72 hours after intravenous injection of Ad.GFP or Ad.IL-6 (1-way ANOVA, P = .001; *P = .007: Alk2fl/fl mice injected with Ad.GFP vs Alk2fl/fl; Alb-Cre mice injected with Ad.GFP; #P = .01: Alk2fl/fl injected with Ad.GFP vs Alk2fl/fl injected with Ad.IL-6; †P = .001: Alk2fl/fl; Alb-Cre mice injected with Ad.GFP vs Alk2fl/fl; Alb-Cre mice injected with Ad.IL-6). (C) Serum iron concentrations were measured in Alk3fl/fl and Alk3fl/fl; Alb-Cre mice injected with Ad.GFP or Ad.IL-6 (1-way ANOVA, P = .0016; *P = .0004: Alk3fl/fl mice injected with Ad.GFP [n = 4] vs Alk3fl/fl; Alb-Cre mice injected with Ad.GFP [n = 6]; #P = .0445: Alk3fl/fl mice injected with Ad.GFP vs Alk3fl/fl mice injected with Ad.IL-6 [n = 4]). Serum iron concentrations in Alk3fl/fl; Alb-Cre mice injected with Ad.GFP did not differ from those in Alk3fl/fl; Alb-Cre mice injected with Ad.IL-6 (P = .6). (D) Transferrin saturations in Alk3fl/fl and Alk3fl/fl; Alb-Cre mice 72 hours after injection with Ad.GFP or Ad.IL-6 (1-way ANOVA, P = .0004; *P = .0001: Alk3fl/fl mice injected with Ad.GFP vs Alk3fl/fl; Alb-Cre mice injected with Ad.GFP; #P = .004: Alk3fl/fl mice injected with Ad.GFP vs Alk3fl/fl mice injected with Ad.IL-6; †P = .003: Alk3fl/fl; Alb-Cre mice injected with Ad.GFP vs Alk3fl/fl; Alb-Cre mice injected with Ad.IL-6).
