Gene expression analysis by RNA-seq demonstrates dysregulation of multiple genes associated with myeloid cell development. (A) Hierarchical cluster analysis generated by Euclidean distance and pairwise complete-linkage analysis of unsupervised RNA-seq data. (B) Heat map (left) and Gene Set Enrichment Analysis (right) show genes related to myeloid and T-lymphoid cell development are up-regulated (red) in sorted AML cells (B220⁺Gr1⁺Mac1⁺) compared with sorted normal myeloid cells (Gr1⁺Mac1⁺) from normal BALB/c mice (P < .05). (C) Leading-edge analysis of RNA-seq data sets demonstrates gene expression patterns in leukemic cells characteristic of both myeloid and T-lymphoid lineage cells. (D) Quantitative RT-PCR analysis confirms the transcription level changes for selected genes in AML cells measured by RNA-seq shown in (B). (E) Schematic summary of differentially expressed genes related to stages of myeloid development.