The VWF CTCK dimer crystal structure. (A-B) Two ribbon diagram views with each monomer in a different color and an inset showing another view of the β7 strands. Disulfides are shown in stick and colored as in Figure 1. (C-D) EM class averages with data from.13 (C) The VWF dimeric bouquet (arrow) in a C-terminal fragment extending from the A3 to the CTCK domain. (D) Class averages of the same fragment, recalculated with centering on the CTCK domain. Scale bars are 10 nm in A and 5 nm in D. A schematic interpretation is shown to right. (E) Experimental electron density around disulfides as in Figure 1E-F. Mesh is contoured at 1σ around cysteine sidechains and is shown in black for inter-chain disulfides and in purple for intra-chain disulfides.
