The effect of Jmjd1c KD on growth and differentiation of LSK cells. (A) Experimental overview. (B) Relative expression of Jmjd1c mRNA in Lin^–Sca-1^–c-Kit⁺ cells transduced with nontargeting control (shScr) or Jmjd1c-targeting shRNA (sh868) at day 2 after transduction. These cells were sorted in parallel with LSK cells used in the experiment. (C) Proliferation of shScr- and shJmjd1c-transduced LSK cells in liquid culture. (D) Number of hematopoietic colonies generated by shScr- and shJmjd1c-transduced LSK cells in semisolid media supplemented with stem cell factor (SCF), interleukin-6 (IL-6), IL-3 and erythropoietin (EPO). (E) Flow cytometry analysis of lineage markers c-Kit and Sca-1 expression in Jmjd1c-depleted and control cells 14 days after sort. (F) Number of erythroid colonies generated by shScr- and shJmjd1c-transduced LSK cells in semisolid culture supplemented with EPO. (G) Erythroid colony morphology generated by shScr- or shJmjd1c-transduced cells. (H) Flow cytometry analysis of Ter119 and CD71 expression in cultures generated in (F).