BTLA downregulation during activation course of Vγ9Vδ2 T cells. (A) Gating strategy showing a representative expression profile of BTLA expression before and after 24 hours in culture with 200 UI/mL IL-2±BrHPP (1 μM). (B) Kinetic analysis of the intensity of BTLA expression (squares, n = 10) and TCR expression (circles, n = 7) after stimulation by IL-2 alone (closed symbols) or in combination with 1 μM BrHPP. (C) Kinetic analysis of BTLA after stimulation by IL-2 (circles, 200 UI/mL), IL-7 (triangles, 25 ng/mL), or IL-15 (diamonds, 10 ng/mL) in the presence (open symbols) or absence (closed symbols) of BrHPP (1 μM) (n = 3). Data represent mean ± SEM of BTLA or TCR Vδ2 MFI.