Repeated intraperitoneal doses of the TNFR superfamily agonist antibodies DTA-1 and OX86 cause anaphylaxis. C57BL/6J mice were treated with 1 mg of (A) DTA-1, (B) OX86, (C) anti-4-1BB (clone LOB.12), or (D) anti-CD40 (clone FGK45.5) on days 0, 4, and 11. Rectal temperatures of individual mice were monitored for 1 hour following the final injection, represented by individual lines in the graphs. Data are representative of 3 independent experiments (n = 5 mice per group).